HCK antibody (truncated)

Catalog No. ABIN2464063

This Mouse Monoclonal antibody specifically detects HCK in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HCK antibody (truncated) (ABIN2464063)

Target: HCK (Hemopoietic Cell Kinase (HCK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 3D12E10

Product Details anti-HCK Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant HCK-His fusion protein is expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: HCK monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for HCK

Target: HCK (Hemopoietic Cell Kinase (HCK))

Alternative Name: HCK

Background: Hemopoietic cell kinase.The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Gene ID: 3055

UniProt: P08631

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling