Lipase A antibody (truncated)

Catalog No. ABIN2464075

This Mouse Monoclonal antibody specifically detects Lipase A in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Lipase A antibody (truncated) (ABIN2464075)

Target: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lipase A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 9G7F12

Product Details anti-Lipase A Antibody

Binding Specificity: truncated

Purification: Antibody is purified by protein A affinity chromatography.

Immunogen: Ni-NTA purified truncated recombinant LAL expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2a

Application Details

Application Notes: Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS containing 0.03 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: LAL monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for Lipase A

Target: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Alternative Name: LAL

Background: Lysosomal acid lipase (LAL), with 378-amino acid protein( 43-54 kDa), functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides which are taken up by receptor-mediated endocytosis. An inherited deficiency or low activity of human lysosomal acid lipase results in the intralysosomal storage of the respective lipid substrates. So it is also responsible for the rare conditions of Wolman disease and cholesteryl ester storage disease (CESD). As the enzyme is synthesized by all nucleated cells, lipid-laden cells are found in all organs, particularly in liver, spleen, the adrenal and the hemopoietic system, and in the intestine as well as in the lymph nodes, lungs, testes, and ovaries.

Gene ID: 3988

UniProt: P38571