Ret Proto-Oncogene antibody (AA 896-1063)

Catalog No. ABIN2464098

The Mouse Monoclonal anti-Ret Proto-Oncogene antibody (Clone 8D10C9) (ABIN2464098) specifically detects Ret Proto-Oncogene in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for Ret Proto-Oncogene antibody (AA 896-1063) (ABIN2464098)

Target: Ret Proto-Oncogene (RET)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ret Proto-Oncogene antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 8D10C9

Product Details anti-Ret Proto-Oncogene Antibody

Binding Specificity: AA 896-1063

Immunogen: Purified truncated recombinant RET (aa896-1063)-GST fusion protein is expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: RET monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for Ret Proto-Oncogene

Target: Ret Proto-Oncogene (RET)

Alternative Name: RET

Background: RET (ret proto-oncogene) is a member of the cadherin superfamily and a receptor tyrosine kinase, which are cell-surface molecules that transduce signals for cell growth and differentiation. It can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.

Gene ID: 5979

UniProt: P07949

Pathways: RTK Signaling, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation