RPS6KA1 antibody (truncated)

Catalog No. ABIN2464100

The Mouse Monoclonal anti-RPS6KA1 antibody is suitable to detect RPS6KA1 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for RPS6KA1 antibody (truncated) (ABIN2464100)

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPS6KA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 10B1D7A9-10B1D7E12

Product Details anti-RPS6KA1 Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant RSK1 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2a

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: RSK1 monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Alternative Name: RSK1

Background: Rsk1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK proteins possess two separate kinase domains, the C terminal kinase domain and the N terminal kinase domain, separated by a linker region containing a hydrophobic motif. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation.

Gene ID: 6195

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades