TYRO3
Reactivity: Human
ELISA
Host: Mouse
Monoclonal
10E11
unconjugated
Application Notes
Western Blot:1:500 - 1:2,000 IF:1:50 - 1:200 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Buffer
Ascitic fluid containing 0.03 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage
-20 °C
Storage Comment
TYRO3 monoclonal antibody can be stored at -20 °C, stable for one year.
Target
TYRO3
(TYRO3 Protein Tyrosine Kinase (TYRO3))
Alternative Name
TYRO3
Background
Tyrosine-protein kinase (TYRO3) belongs to the Tyr protein kinase family (AXL/UFO subfamily). The UFO family of receptor tyrosine kinases is comprised of subfamily members Rse(also referred to as Tyro3 or Sky) and UFO (also called Tyro7 or Axl). Two distinct isoforms of Rse, designated Brt and Etk-2, have been described. Brt differs from Rse at its C-terminus, but more importantly lacks the N-terminal 31 amino acid signal peptide sequence present in Rse, which is replaced by a 27 amino acid Brt-specific sequence. It has been suggested that as a result of this alternative splicing event, Brt resides in the cytoplasm, unlike Rse which is expressed on the cell surface. Ekt-2 also lacks an N-terminal signal peptide which is substituted with a 45 amino acid Ekt-2-specific sequence. Protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.