JUP antibody (C-Term)

Catalog No. ABIN2465235

The Goat Polyclonal anti-JUP antibody (ABIN2465235) specifically detects JUP in WB, ELISA and EIA. The antibody is reactive with Human samples.

Quick Overview for JUP antibody (C-Term) (ABIN2465235)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Enzyme Immunoassay (EIA)

Product Details anti-JUP Antibody

Binding Specificity: C-Term

Specificity: This antibody is expected to recognize both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Purification: JUP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: JUP antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of JUP.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 85 kDa band observed in A431 lysate (predicted MW of 86 kDa according to NP_002221, NP_068831). Recommended for use at 0.2-1 μg/mL. Enzyme immunoassay: Customer finds it successful in quantitative ELISA assays (EIA) but the antibody is very sensitive to freeze/thaw cycles in this assay.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP

Gene ID: 3728

NCBI Accession: NP_002221, NP_068831

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location