SAG antibody (Internal Region)

Catalog No. ABIN2465438

The Goat Polyclonal anti-SAG antibody has been validated for WB, ELISA and IP. It is suitable to detect SAG in samples from Human, Mouse and Rat.

Quick Overview for SAG antibody (Internal Region) (ABIN2465438)

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SAG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-SAG Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognize both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.

Purification: Arrestin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Arrestin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Arrestin.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa band observed in human brain lysates (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows mainly nuclear staining of Purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for SAG

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Alternative Name: Arrestin

Gene ID: 409

NCBI Accession: NP_004304, NP_945355

UniProt: P32121

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling