APAF1 antibody (AA 307-388)

Catalog No. ABIN2467772

The Chicken Polyclonal anti-APAF1 antibody is suitable to detect APAF1 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for APAF1 antibody (AA 307-388) (ABIN2467772)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APAF1 Antibody

Binding Specificity: AA 307-388

Purification: Antigen affinity-purified

Application Details

Application Notes: Apaf-1 antibody can be used for the detection of Apaf-1 by Western blot, may also work for IHC and ICC.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline. No preservatives added.

Preservative: Without preservative

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C/-20 °C

Storage Comment: APAF1 antibody can be stored at 4 °C for short term (weeks). Long term storage should be at -20 °C.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1

Background: Apoptotic protease activating factor 1. Apaf-1 is a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Molecular Weight: 141.8 kDa (calculated)

Gene ID: 317

NCBI Accession: NP_863658

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity