JAG1 antibody (AA 110-125)

Catalog No. ABIN2470551

This anti-JAG1 antibody is a Rabbit Polyclonal antibody detecting JAG1 in WB, ELISA, IHC, ICC and IF. Suitable for Human.

Quick Overview for JAG1 antibody (AA 110-125) (ABIN2470551)

Target: JAG1 (Jagged 1 (JAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-JAG1 Antibody

Binding Specificity: AA 110-125

Purification: Protein A Column

Immunogen: JAG1 antibody was raised against a synthetic peptide corresponding to aa 110 - 125 of JAG1 (Human).

Isotype: IgG

Application Details

Application Notes: JAG1 antibody can be used in Western Blot starting at 1:500 - 1:1000, immunohistochemistry starting at 5 μg/mL, and immunocytochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store JAG1 antibody at 4 °C or -20 °C.

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: JAG1

Background: Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome. A BLAST analysis was used to suggest crossreactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100 % homology with the immunizing sequence. Partial reactivity is expected against canine (81 %) and Xenopus laevis (85 %) based on partial sequence homologies as indicated. The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

Gene ID: 182

UniProt: P78504

Pathways: Notch Signaling, Stem Cell Maintenance