Actin antibody (N-Term)

Catalog No. ABIN2471523

This anti-Actin antibody is a Mouse Monoclonal antibody detecting Actin in WB, ELISA and IHC. Suitable for Human, Rat, Rabbit, Pig and Goat.

Quick Overview for Actin antibody (N-Term) (ABIN2471523)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Rat, Rabbit, Pig, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3B3

Product Details anti-Actin Antibody

Binding Specificity: N-Term

Specificity: Highly specific for alpha- skeletal actin, and does not cross react with other actin isoforms. The epitope recognized by 3B3 is highly conserved. Therefore the antibody cross-reacts with many other species.

Purification: Purified

Immunogen: Peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue

Isotype: IgG1

Application Details

Application Notes: ACTA1 Monoclonal Antibody can be used for immunohistochemistry (tested on frozen tissues) starting at 1:100 - 1:1000, immunohistochemistry (tested on parafin embedded tissues) starting at 10 μg/mL and Western Blot starting at 1:1000 - 1:5000.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freezing.

Storage: 4 °C

Storage Comment: ACTA1 Monoclonal antibody may be stored at +4 °C, avoid freezing

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Gene ID: 58

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development