CD4 antibody (PE)

Catalog No. ABIN2480170

This Mouse Monoclonal antibody specifically detects CD4 in FACS. It exhibits reactivity toward Cowand has been mentioned in 1 publication.

Quick Overview for CD4 antibody (PE) (ABIN2480170)

Target: CD4

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: CC8

Product Details anti-CD4 Antibody (PE)

Characteristics: Mouse anti Bovine CD4 antibody, clone CC8 recognizes bovine CD4, the homolog of human CD4 and immunoprecipitates a ~50 kDa molecule. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Clone CC8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Eskra et al. 1991).

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Immunogen: Bovine lymphocytes

Isotype: IgG2a

Application Details

Application Notes: Flow: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 ml distilled water

Buffer: Phosphate buffered saline, 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin, 5% Sucrose

Handling Advice: DO NOT FREEZE This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage Comment: Store at +4°C. This product should be stored undiluted.

References for anti-CD4 antibody (PE) (ABIN2480170)

Endsley, Furrer, Endsley, McIntosh, Maue, Waters, Lee, Estes: "Characterization of bovine homologues of granulysin and NK-lysin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 4, pp. 2607-14, (2004) (PubMed).

Target Details for CD4

Target: CD4

Alternative Name: CD4

UniProt: A7YY52

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events