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SOD1 antibody (FITC)

The Rabbit Polyclonal anti-SOD1 antibody has been validated for WB, IHC, ELISA, IP, ICC and IF. It is suitable to detect SOD1 in samples from Rat.
Catalog No. ABIN2481590

Quick Overview for SOD1 antibody (FITC) (ABIN2481590)

Target

See all SOD1 Antibodies
SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity

  • 190
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Rat

Host

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Rabbit

Clonality

  • 220
  • 64
Polyclonal

Conjugate

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This SOD1 antibody is conjugated to FITC

Application

  • 248
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
  • Specificity

    Detects ~23 kDa (human) and ~19 kDa (other species).

    Cross-Reactivity

    Cow, Fusarium, Human, Mouse, Rat

    Purification

    Protein A Purified

    Immunogen

    Synthetic Rat Cu/Zn SOD Peptide
  • Application Notes

    • WB (1:2000)
    • ICC/IF (1:120)
    • optimal dilutions for assays should be determined by the user.

    Comment

    0.5 μg/ml of ABIN2481590 was sufficient for detection of Cu/Zn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    PBS pH 7.0, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Conjugated antibodies should be stored at 4°C
  • Target

    SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

    Alternative Name

    SOD1

    Background

    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

    Gene ID

    24786

    NCBI Accession

    NP_058746

    UniProt

    P07632

    Pathways

    Sensory Perception of Sound, Transition Metal Ion Homeostasis
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