Calnexin antibody (C-Term) (Atto 594)

Catalog No. ABIN2481633

This anti-Calnexin antibody is a Rabbit Polyclonal antibody detecting Calnexin in WB, IHC, IF, IP, FACS and ICC. Suitable for Dog.

Quick Overview for Calnexin antibody (C-Term) (Atto 594) (ABIN2481633)

Target: Calnexin (CANX)

Reactivity: Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Calnexin Antibody (Atto 594)

Binding Specificity: C-Term

Specificity: Detects the C-terminal domain of Calnexin ~90 kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.

Cross-Reactivity: Chicken, Cow, Dog, Drosophila melanogaster, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Quail, Rabbit, Rat, Sheep, Xenopus laevis

Purification: Protein A Purified

Immunogen: Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Application Details

Application Notes:

• WB (1:2000)
• ICC/IF (1:100)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:2000 dilution of ABIN2481633 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Gene ID: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: MAPK Signaling, Thyroid Hormone Synthesis