NALCN antibody (AA 1659-1738) (Atto 594)

Catalog No. ABIN2483022

This Mouse Monoclonal antibody specifically detects NALCN in WB, ICC and IF. It exhibits reactivity toward Rat.

Quick Overview for NALCN antibody (AA 1659-1738) (Atto 594) (ABIN2483022)

Target: NALCN (Sodium Leak Channel, Non-Selective (NALCN))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NALCN antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S187-7

Product Details anti-NALCN Antibody (Atto 594)

Binding Specificity: AA 1659-1738

Specificity: Detects ~200 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483022 was sufficient for detection of NALCN in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for NALCN

Target: NALCN (Sodium Leak Channel, Non-Selective (NALCN))

Alternative Name: NALCN

Background: NALCN (sodium leak channel non-selective protein), alsoknown as CanIon or VGCNL1 (voltage gated channel-like protein 1), is a 1738 amino acid multi-pass membrane protein that belongs to the cation-nonselective channel family. NALCN is highly conserved in mammals and is widely expressed in the central nervous system. Activated by NK-1R, NALCN is a voltage-independent, nonselective cation channel which is permeable to sodium, potassium and calcium ions. NALCN is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous systems. Defects of NALCN in mice causes disruption in respiratory rhythm and death occurs within 24 hours of birth. Three isoforms of NALCN exists due to alternative splicing events.

Gene ID: 266760

NCBI Accession: NP_705894

UniProt: Q6Q760