MDC1 antibody (N-Term) (HRP)

Catalog No. ABIN2484153

This Mouse Monoclonal antibody specifically detects MDC1 in WB, IF and ICC. It exhibits reactivity toward Mouse.

Quick Overview for MDC1 antibody (N-Term) (HRP) (ABIN2484153)

Target: MDC1 (Mdc1 (MDC1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MDC1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: P2B11

Product Details anti-MDC1 Antibody (HRP)

Binding Specificity: N-Term

Specificity: Detects ~184 kDa. This antibody recognizes MDC1 at and around the N-terminus.

Cross-Reactivity: Chimpanzee, Cow, Human, Mouse

Purification: Protein G Purified

Immunogen: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

Isotype: IgG1

Application Details

Application Notes:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2484153 was sufficient for detection of MDC1 in 10 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1

Background: MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.

Gene ID: 240087

NCBI Accession: NP_001010833

UniProt: Q5PSV9