DNMT1 antibody (AA 637-650) (Biotin)

Catalog No. ABIN2486005

The Mouse Monoclonal anti-DNMT1 antibody has been validated for WB, IHC, IP, ChIP. It is suitable to detect DNMT1 in samples from Human.

Quick Overview for DNMT1 antibody (AA 637-650) (Biotin) (ABIN2486005)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 60B1220-1

Product Details anti-DNMT1 Antibody (Biotin)

Binding Specificity: AA 637-650

Specificity: Detects ~180 kDa. It will cross-react with mouse DNMT1.

Cross-Reactivity: Fish, Human, Mouse, Zebrafish (Danio rerio)

Purification: Protein G Purified

Immunogen: Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

2 μg/ml of ABIN2486005 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Gene ID: 1786

NCBI Accession: NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection