SOD1 antibody (N-Term) (HRP)

Catalog No. ABIN2486245

The Rabbit Polyclonal anti-SOD1 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect SOD1 in samples from Human.

Quick Overview for SOD1 antibody (N-Term) (HRP) (ABIN2486245)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SOD1 Antibody (HRP)

Binding Specificity: N-Term

Specificity: Recognizes a conformation specific epitope where the beta barrel is unfolded.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Within 50 AA of N-terminal region of SOD1, unfolded beta barrel region

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1μg/ml of ABIN2486245 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.

Gene ID: 6647

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis