Aquaporin 4 antibody (C-Term) (Biotin)

Catalog No. ABIN2486394

This Rabbit Polyclonal antibody specifically detects Aquaporin 4 in WB, IHC, ICC and IF. It exhibits reactivity toward Rat.

Quick Overview for Aquaporin 4 antibody (C-Term) (Biotin) (ABIN2486394)

Target: Aquaporin 4 (AQP4)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Aquaporin 4 Antibody (Biotin)

Binding Specificity: C-Term

Specificity: Detects ~35 kDa. May detect larger glycosylated bands ~50 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4

Application Details

Application Notes:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2486394 was sufficient for detection of aquaporin 4 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: Aquaporin 4

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). It is also expressed in astrocytes and is up regulated by direct insult to the CNS (4). Aquaporin 4 has been proposed as the primary autoimmune target in neuromyelitis optica ( 5, 6).

Gene ID: 25293

NCBI Accession: NP_036957

UniProt: P47863

Pathways: Sensory Perception of Sound