PCK1 antibody

Catalog No. ABIN249350

The Rabbit Polyclonal anti-PCK1 antibody has been validated for WB. It is suitable to detect PCK1 in samples from Mouse, Zea mays, Green bean and Miscanthus giganteus. There is 1 publication available.

Quick Overview for PCK1 antibody (ABIN249350)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Mouse, Zea mays, Green bean, Miscanthus giganteus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCK1 Antibody

Cross-Reactivity (Details): No cross-reactivity with: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 73 / 78 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB) MW 73 | 78 kDa.

Comment:

due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-PCK1 antibody (ABIN249350)

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PEPCK

Background: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) Is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2. Both genes are nuclear, located on chromosome 4 and 5 respectively (in Arabidopsis). The protein products, PEPCK1 and PEPCK2, are both highly conserved in the model plant, Arabidopsis thaliana.

Molecular Weight: expected: 73 kDa, apparent: 78 kDa

UniProt: Q9SLZ0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis