PCK1 antibody

Catalog No. ABIN249350

There is 1 publication for this product available. The Rabbit Polyclonal anti-PCK1 antibody is suitable to detect PCK1 in samples from Mouse, Zea mays, Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum virgatum, Miscanthus giganteus, Nannochloropsis oceanica, Setaria viridis, Spartina alterniflora, Spartina patens and Green bean. It has been validated for WB.

Quick Overview for PCK1 antibody (ABIN249350)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Mouse, Zea mays, Saccharum, Pineapple, Oryza sativa, Megathyrsus maximus, Panicum virgatum, Miscanthus giganteus, Nannochloropsis oceanica, Setaria viridis, Spartina alterniflora, Spartina patens, Green bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCK1 Antibody

Purpose: Anti-PEPCK | PEP carboxykinase

Cross-Reactivity (Details): Ananas comosus, Miscantus giganteus, Mouse, Nannochloropsis oceanica, Oryza sativa, Panicum maximum, Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301,Setaria viridis, Spartina alterniflora, Spartina patens, Zea mays
Not reactive in: Arabidopsis thaliana, Pinus yunnanensis

Predicted Reactivity: Brassica napus,Chromera velia,Cucumis sativus,Flaveria sp.,Lycopersicon esculentum,Medicago sativa,Oryza sativa,Urochloa panicoides,Zoysia japonica,Zea mays

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Application Details

Application Notes: 1 : 1000 (WB)

Comment:

due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-PCK1 antibody (ABIN249350)

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PEPCK

Background: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.

Molecular Weight: 73 | 78 kDa

Gene ID: 541622

UniProt: Q9SLZ0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis