ATP6AP1 antibody

Catalog No. ABIN249357

This anti-ATP6AP1 antibody is a Rabbit Polyclonal antibody detecting ATP6AP1 in WB, IF and IL. Suitable for Arabidopsis thaliana, Chlamydomonas and Soybean. This Primary Antibody has been cited in 8+ publications.

Quick Overview for ATP6AP1 antibody (ABIN249357)

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Reactivity: Arabidopsis thaliana, Chlamydomonas, Soybean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6AP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunolocalization (IL)

Product Details anti-ATP6AP1 Antibody

Cross-Reactivity: Soybean (Glycine max)

Cross-Reactivity (Details): No cross-reactivity with: Aspergillus niger

Characteristics: Expected / apparent Molecular Weight of the Antigene: 95 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Application Details

Application Notes: Recommended Dilution: 1 : 1000 - 1 : 5000 with standard ECL OR 1 : 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1 : 600 - 1 : 1000 (IF).
Cellular [compartment marker] for plasma membrane

Comment:

for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Do not store this antibody at 4 °C.

References for anti-ATP6AP1 antibody (ABIN249357)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Chen, Fujii, Yamaji, Masuda, Takemoto, Kamiya, Yusuyin, Iwasaki, Kato, Maeshima, Ma, Ueno: "Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family." in: Journal of experimental botany, Vol. 64, Issue 14, pp. 4375-87, (2013) (PubMed).

Menckhoff, Mielke-Ehret, Buck, Vuletić, Lüthje: "Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: Native versus recombinant protein." in: Journal of proteomics, Vol. 80C, pp. 66-77, (2013) (PubMed).

Visnovitz, Solti, Csikós, Fricke: "Plasma membrane H(+) -ATPase gene expression, protein level and activity in growing and non-growing regions of barley (Hordeum vulgare) leaves." in: Physiologia plantarum, Vol. 144, Issue 4, pp. 382-93, (2012) (PubMed).

Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

Ito, Batth, Petzold, Redding-Johanson, Mukhopadhyay, Verboom, Meyer, Millar, Heazlewood: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).

Chen, Xiao, Wu, Dong, He, Pei, Zheng: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." in: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, (2010) (PubMed).

Page, Kropat, Hamel, Merchant: "Two Chlamydomonas CTR copper transporters with a novel cys-met motif are localized to the plasma membrane and function in copper assimilation." in: The Plant cell, Vol. 21, Issue 3, pp. 928-43, (2009) (PubMed).

Target Details for ATP6AP1

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Alternative Name: H+ATPase

Background: AGI Code: At2g18960
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Molecular Weight: 95 kDa (Arabidopsis thaliana)

UniProt: P20649

Pathways: Proton Transport, SARS-CoV-2 Protein Interactome