ATP6AP1 antibody (Internal Region)

Catalog No. ABIN6260131

Product Details anti-ATP6AP1 Antibody

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6AP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ATP6AP1 Antibody detects endogenous levels of total ATP6AP1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATP6AP1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP6AP1

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Alternative Name: ATP6AP1 (ATP6AP1 Products)

Synonyms: 16A antibody, ATP6IP1 antibody, ATP6S1 antibody, Ac45 antibody, CF2 antibody, VATPS1 antibody, XAP-3 antibody, XAP3 antibody, AC45 antibody, AI316502 antibody, AW108110 antibody, Atp6ip1 antibody, Atp6s1 antibody, C7-1 antibody, mFLJ00383 antibody, H+-ATPase antibody, ATPase H+ transporting accessory protein 1 antibody, ATPase, H+ transporting, lysosomal accessory protein 1 antibody, plasma membrane ATPase 4 antibody, ATP6AP1 antibody, Atp6ap1 antibody, LOC547525 antibody

Background:

Description: Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca2+-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe2+ prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633).

Gene: ATP6AP1

Molecular Weight: 52kDa

Gene ID: 537

UniProt: Q15904

Pathways: Proton Transport, SARS-CoV-2 Protein Interactome