ATP6AP1 antibody (Middle Region)

Catalog No. ABIN2782118

The Rabbit Polyclonal anti-ATP6AP1 antibody is suitable to detect ATP6AP1 in samples from Human, Mouse, Rat, Dog, Horse, Rabbit and Guinea Pig. It has been validated for WB.

Quick Overview for ATP6AP1 antibody (Middle Region) (ABIN2782118)

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6AP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6AP1 Antibody

Binding Specificity: Middle Region

Sequence: SPVIHPPVSY NDTAPRILFW AQNFSVAYKD QWEDLTPLTF GVQELNLTGS

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ATP6AP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP6AP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 470 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP6AP1

Target: ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))

Alternative Name: ATP6AP1

Background: This gene encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.
Alias Symbols: 16A, ATP6IP1, ATP6S1, Ac45, CF2, MGC129781, VATPS1, XAP-3, XAP3
Protein Interaction Partner: K3, NR1I2, UBC, nef, HNRNPAB, ATP6V1C1, FBXO6, SYVN1, HBA2, ANG, HBA1,
Protein Size: 470

Molecular Weight: 52 kDa

Gene ID: 537

NCBI Accession: NM_001183, NP_001174

UniProt: Q15904

Pathways: Proton Transport, SARS-CoV-2 Protein Interactome