DHRS2 antibody (AA 61-160) (HRP)

Catalog No. ABIN2559684

The Rabbit Polyclonal anti-DHRS2 antibody is suitable to detect DHRS2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and IHC (fro).

Quick Overview for DHRS2 antibody (AA 61-160) (HRP) (ABIN2559684)

Target: DHRS2 (Dehydrogenase/reductase (SDR Family) Member 2 (DHRS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHRS2 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DHRS2 Antibody (HRP)

Binding Specificity: AA 61-160

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DHRS2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DHRS2

Target: DHRS2 (Dehydrogenase/reductase (SDR Family) Member 2 (DHRS2))

Alternative Name: DHRS2

Background:

Synonyms: HEP27, SDR25C1, Dehydrogenase/reductase SDR family member 2, mitochondrial, Dicarbonyl reductase HEP27, Protein D, DHRS2

Background: Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.

Gene ID: 10202

UniProt: Q13268

Pathways: C21-Steroid Hormone Metabolic Process