CD25 antibody (APC)

Catalog No. ABIN2567382

The Rat Monoclonal anti-CD25 antibody has been validated for FACS. It is suitable to detect CD25 in samples from Mouse.

Quick Overview for CD25 antibody (APC) (ABIN2567382)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: PC61-5

Product Details anti-CD25 Antibody (APC)

Specificity: The PC61.5 antibody specifically reacts with mouse CD25, the 55 kDa low-affinity Interleukin-2 Receptor α chain (IL-2R α), expressed on early progenitors of T and B lineage, and on B and T cells.

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Isotype: IgG1 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage: 4 °C

Storage Comment: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: The PC61.5 antibody specifically reacts with mouse CD25, the 55 kDa low-affinity Interleukin-2 Receptor α chain (IL-2R α), expressed on early progenitors of T and B lineage, and on B and T cells. Together with CD122 (IL-2 Receptor β) and CD 132 (IL-2 Receptor γc, the common gamma chain), CD25 forms high-affinity receptor complexes for IL-2. Resting B and T cells and natural killer cells do not express IL-2Rα. Cd25 is also expressed on the dendritic cells, and it enhances lymphocyte differentiation and activation.The PC61.5 antibody block the binding of IL-2 to both high-affinity and low-affinity receptors.

Gene ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation