CD4 antibody (violetFluor™ 450)

Catalog No. ABIN2567668

This anti-CD4 antibody is a Rat Monoclonal antibody detecting CD4 in FACS. Suitable for Mouse.

Quick Overview for CD4 antibody (violetFluor™ 450) (ABIN2567668)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to violetFluor™ 450

Application: Flow Cytometry (FACS)

Clone: RM4-5

Product Details anti-CD4 Antibody (violetFluor™ 450)

Specificity: The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells.

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage: 4 °C

Storage Comment: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: The RM4-5 monoclonal antibody specifically reacts with mouse CD4, also known as L3T4, a 55 kDa differentiation antigen expressed by the majority of thymocytes, subpopulations of mature T cells (like major histocompatibility complex class II-restricted T lymphocytes), a subset of natural killer T cells, and on pluripotent hematopoietic stem cells. CD4 binds to the major histocompatibility complex class II (MHC class II) and enhances T lymphocyte development and mature T cells functions. In T lymphocytes, CD4 binds to the cytoplasmic tail of enzyme tyrosine kinase (p56lck).Binding of RM4-5 is blocked by the anti-mouse CD4 clone GK1.5.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Gene ID: 12504

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events