CD4 antibody (Internal Region)
Quick Overview for CD4 antibody (Internal Region) (ABIN2613402)
Target
See all CD4 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- Internal Region
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Purpose
- CD4
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Sequence
- KNKEVSVKRV TQDPK
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Specificity
- This antibody is expected to recognize isoform 1 (NP_000607.1) and isoform 2 (NP_001181943.1).
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Cross-Reactivity
- Human
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Isotype
- IgG
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Rabbit, Cat, Cow, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB, IHC Host: Goat Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 Reactivity: Human FACS, WB, MS Host: Mouse Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (AA 26-200) antibodyCD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC
anti-CD4 (CD4) antibody (PE)CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE
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Application Notes
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 4 μg/mL.
Western Blot: Approx 30 kDa band observed in Human Spleen and Bone Marrow lysates (calculated MW of 31.3 kDa according to NP_001181943.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:128000.
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Comment
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Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Minimize freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- CD4
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Alternative Name
- CD4
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Background
- CD4, CD4 Molecule, CD4mut, CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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Gene ID
- 920
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NCBI Accession
- NP_000607, NP_001181943
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Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Target
-
