Cyclin D1 antibody (Internal Region)

Catalog No. ABIN2613417

The Goat Polyclonal anti-Cyclin D1 antibody is suitable to detect Cyclin D1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Cyclin D1 antibody (Internal Region) (ABIN2613417)

Target: Cyclin D1 (CCND1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Cyclin D1 Antibody

Binding Specificity: Internal Region

Purpose: cyclin D1

Sequence: TRFLSRVIKC DPD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human Placenta lysates (calculated MW of 33.7 kDa according to NP_444284.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1

Background: CCND1, cyclin D1, BCL1, D11S287E, PRAD1, U21B31, B-cell CLL/lymphoma 1, B-cell lymphoma 1 protein, BCL-1 oncogene, G1/S-specific cyclin-D1, PRAD1 oncogene

Gene ID: 595

NCBI Accession: NP_444284

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling