ICAM1 antibody (AA 313-327)

Catalog No. ABIN2613438

The Goat Polyclonal anti-ICAM1 antibody has been validated for WB, FACS, ELISA and IF. It is suitable to detect ICAM1 in samples from Human.

Quick Overview for ICAM1 antibody (AA 313-327) (ABIN2613438)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-ICAM1 Antibody

Binding Specificity: AA 313-327

Purpose: ICAM1 (aa313-327)

Sequence: PNVILTKPEV SEGTE

Specificity: The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite..

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Human Lung lysates (calculated MW of 57.8 kDa according to Human NP_000192.2).This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells and Human peripheral blood monocytes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM1

Background: ICAM1, intercellular adhesion molecule 1, BB2, CD54, P3.58, ICAM-1, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor

Gene ID: 3383

NCBI Accession: NP_000192

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling