ABCB1 antibody

Catalog No. ABIN2613685

This anti-ABCB1 antibody is a Mouse Monoclonal antibody detecting ABCB1 in WB, IHC, IHC (p), FACS, IP, IHC (fro) and ICC. Suitable for Human, Mouse, Rat and Hamster.

Quick Overview for ABCB1 antibody (ABIN2613685)

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ABCB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: C219

Product Details anti-ABCB1 Antibody

Specificity: Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein, not species specific. Species Crossreactivity: human, mouse, rat and hamster. Crossreactivity Proteins: a ~200kD protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2).

Purification: Protein A purified

Immunogen: SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.

Type of Immunogen: Cell fraction

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, IP, WB (1:25 - 1:50)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Cell permeabilization required. Western Blot: 1:25-1:50. Immunohistochemistry (Paraffin and Frozen): For paraffin sections, dilute 1:12.5 for biotin based detection. The antibody should be incubated for 60 minutes at RT. Immunocytochemistry: Cytospin preparations. Positive control: Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.

Comment:

Target Species of Antibody: Hamster

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Alternative Name: ABCB1 / MDR1 / P Glycoprotein

Background: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

Gene ID: 5243

UniProt: P08183