AIF antibody (C-Term)

Catalog No. ABIN2630015

This Goat Polyclonal antibody specifically detects AIF in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for AIF antibody (C-Term) (ABIN2630015)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-AIF Antibody

Binding Specificity: C-Term

Purpose: AIFM1

Sequence: NEVAKLFNIH ED

Specificity: This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65 kDa observed in Pig Heart lysates (calculated MW of 66.9 kDa according to Human NP_004199.1 and 66.8 kDa according

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIFM1

Background: AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor

Gene ID: 9131, 26926, 83533

NCBI Accession: NP_004199, NP_665811, NP_665812, NP_001124318

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect