MYF5 antibody (Internal Region)

Catalog No. ABIN263192

The Goat Polyclonal anti-MYF5 antibody has been validated for WB and ELISA. It is suitable to detect MYF5 in samples from Human.

Quick Overview for MYF5 antibody (Internal Region) (ABIN263192)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MYF5 Antibody

Binding Specificity: Internal Region

Purpose: MYF5

Sequence: PECNSPVWSR KSST

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PECNSPVWSRKSST, from the internal region of the protein sequence according to NP_005584.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4 kDa according to NP_005584.1). Recommended concentration: 0.5-1.5 μg/mL. An additional band of 26 kDa was consistently observed, however this band was not bloc

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: MYF5

Background: MYF5 , myogenic factor 5

Gene ID: 4617

NCBI Accession: NP_005584

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development