Hexokinase 2 antibody (N-Term)

Catalog No. ABIN263918

The Mouse Monoclonal anti-Hexokinase 2 antibody has been validated for WB and EIA. It is suitable to detect Hexokinase 2 in samples from Human.

Quick Overview for Hexokinase 2 antibody (N-Term) (ABIN263918)

Target: Hexokinase 2 (HK2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1A7

Product Details anti-Hexokinase 2 Antibody

Binding Specificity: AA 1-917, N-Term

Specificity: This antibody detects Hexokinase 1, 2.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Hexokinase type II, Muscle form hexokinase, HK2, HKII

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human Hexokinase 2 (1-917aa) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot: 1: 1,000 approx. 1: 3,000, 1: 2,000 as starting dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: Hexokinase-2

Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.Synonyms: HK2, HKII, Hexokinase type II, Muscle form hexokinase

Gene ID: 3099

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect