Prothrombin antibody

Catalog No. ABIN264058

This Mouse Monoclonal antibody specifically detects Prothrombin in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Prothrombin antibody (ABIN264058)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: BDI095

Product Details anti-Prothrombin Antibody

Specificity: This antibody binds Prothrombin, Prethrombin 1 and fragment 2 (residues 156-271) in solid-phase ELISA. Blots Human Prothrombin in Western blot.

Characteristics: Synonyms: Coagulation factor II

Purification: Gel filtration and Ion Exchange Chromatography

Immunogen: Purified Human Prothrombin

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot. A starting range of 1-5g/mL is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled water

Concentration: 1.0 mg/mL (prior to lyophilization)

Buffer: 10 mM Sodium phosphate, 0.15 M Sodium Chloride, pH 7.4 containing 0.01 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one monthor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: Prothrombin (F2)

Background: Prothrombin (Factor 2 in the coagulation cascade) is proteolytically cleaved at two sites by Factor Xa to form thrombin (Factor IIa) in an important step of the coagulation cascade which ultimately results in the stemming of blood loss. Thrombin being the single most important constituent of the coagulation cascade in terms of its feedback activation roles. Prothrombin also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in Prothrombin lead to various forms of thrombosis and dysprothrombinemia.Synonyms: Coagulation factor II

Gene ID: 2147

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling