GAL4 antibody (Activation Domain)

Catalog No. ABIN264526

This Mouse Monoclonal antibody specifically detects GAL4 in WB and IP. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for GAL4 antibody (Activation Domain) (ABIN264526)

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 5C8

Product Details anti-GAL4 Antibody

Binding Specificity: Activation Domain

Specificity: Recognizes GAL4 derivatives containing the C-terminal activation domain.

Characteristics: Synonyms: GAL81, Regulatory protein GAL4

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full lengthGAL4 expressed in E. Coli and mouse myeloma cells.

Isotype: IgG1

Application Details

Application Notes: Western blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAL4

Target: GAL4 (LGALS4) (Galectin 4 (LGALS4))

Alternative Name: GAL4

Background: GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.Synonyms: GAL81, Regulatory protein GAL4

Gene ID: 855828

UniProt: P04386