EPHB1
Reactivity: Human
WB
Host: Sheep
Polyclonal
unconjugated
Application Notes
Western Blot: 1 - 10 μg/mL. Immunoprecipitation: 10 - 20 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The immunogen used for the anti-human ephB1 (Elk receptor) -EXT region was a GST fusion protein ephB1 (Elk receptor) -EXT corresponding to amino acid sequence 16-351 of the N terminus region and having a molecular weight of 116 kD. ephB1, previously known as Elk(eph-like kinase), is a receptor tyrosine kinase of the highly tissue restricted family of eph proteins. EphB1 and other ephB family members are type 1 membrane spanning proteins, comprised of immunoglobulin, fibronectin type III, and cysteine rich subdomains in the ecto domain, and the single uninterrupted cytoplasmic tyrosine kinase domain upstream of a carboxyterminal sterile alpha motif (SAM) domain. ephB family proteins bind ephrins of the B class, ligands that are also transmembrane spanning proteins capable of transmitting signals. ephB1 is expressed predominantly in developing neural structures in embryos, and in vascular epithelium of kidney, and other tissues. Upon binding to alternatively oligomerized ephrin B1, ephB1 signals regulation of cell attachment and cellcell assembly. Members of this protein family are implicated in neuronal and vascular cell targeting.Synonyms: ELK, EPH2, EPHT2, Ephrin type-B receptor 1, HEK6, NET, Tyrosine-protein kinase receptor EPH-2