VAV2 antibody

Catalog No. ABIN264963

The Sheep Polyclonal anti-VAV2 antibody (ABIN264963) specifically detects VAV2 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for VAV2 antibody (ABIN264963)

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-VAV2 Antibody

Specificity: This antibody reacts to Vav-2 DPH.

Purification: Ammonium Sulfate Precipitation

Immunogen: A his tagged fusion protein Vav-2 corresponding to amino acid sequence 287-578 and having a Molecular Weight of 100kD

Isotype: IgG

Application Details

Application Notes: Western Blot: 1: 5,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.08 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: VAV2

Background: Vav-2 has a molecular weight of 100 kD and shares over a 50 % homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetiic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokenesis frequently are uncoupled.Synonyms: Guanine nucleotide exchange factor VAV2, VAV-2

Gene ID: 7410

NCBI Accession: NP_001127870

UniProt: P52735

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway