VAV2
Reactivity: Human, Mouse, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1: 5,000. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS with 0.08 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Vav-2 has a molecular weight of 100 kD and shares over a 50 % homology at the amino acid and nucleic acid level with Vav. Vav-2 is a ubiquitously expressed structural homolog of the Vav protooncogene that is expressed preferentially in hematopoetiic cells. Both proteins are comprised of a Dbl- homology ((DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type vav-2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a Vav-2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokenesis frequently are uncoupled.Synonyms: Guanine nucleotide exchange factor VAV2, VAV-2