AKAP12 antibody (AA 620-1596)

Catalog No. ABIN264964

Product Details anti-AKAP12 Antibody

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Binding Specificity: AA 620-1596

Reactivity: Rat, Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Ammonium Sulfate Precipitation.

Immunogen: Recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein.

Isotype: IgG

Application Details

Application Notes: Western Blot: 2-5 μg/mL. Immunoprecipitation. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: AKAP12 / SSeCKS (AKAP12 Products)

Background: Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicatedduring the process of spermiogenesis and in the actinbased stellate morphology of mesangial cells.

Gene ID: 10116

UniProt: Q62766

Pathways: cAMP Metabolic Process