APAF1 antibody (AA 12-28)

Catalog No. ABIN264991

This Rabbit Polyclonal antibody specifically detects APAF1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for APAF1 antibody (AA 12-28) (ABIN264991)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APAF1 Antibody

Binding Specificity: AA 12-28

Specificity: This antibody reacts to APAF1.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 12 to 28 of the human Apaf-1 protein. The immunogen sequence is identical in human and mouse

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.25 to 0.5 μg/mL. Detects a 130 kDa band in whole HeLa cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline solution containing 0.02 % sodium azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8 °C.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1

Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413

Gene ID: 317

NCBI Accession: NP_001151

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity