SIAH1 antibody
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- Target See all SIAH1 Antibodies
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SIAH1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- This antibody detects SIAH1 / E3 Ubiquitin Ligase. Antibody tested on human brain lysate.
- Purification
- Purified
- Immunogen
- Synthetic peptide derived from the human SIAH-1 protein
- Isotype
- IgG
- Top Product
- Discover our top product SIAH1 Primary Antibody
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- Application Notes
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Western blot (1-5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- Phosphate buffered saline with 0.08 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
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- Target
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
- Alternative Name
- SIAH1 (SIAH1 Products)
- Synonyms
- SIAH antibody, Siah1 antibody, Siah1a antibody, id:ibd2635 antibody, zgc:56026 antibody, SIAH1A antibody, siah antibody, E3 ubiquitin-protein ligase Siah1 antibody, E3 ubiquitin-protein ligase siah-1 antibody, siah E3 ubiquitin protein ligase 1 antibody, siah E3 ubiquitin protein ligase 1 L homeolog antibody, Tsp_03055 antibody, siah-1 antibody, Siah1 antibody, siah1 antibody, SIAH1 antibody, siah1.L antibody
- Background
- Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1. Both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a
- Gene ID
- 6477
- NCBI Accession
- NP_001006611
- UniProt
- Q8IUQ4
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