SIAH1 antibody

Catalog No. ABIN265177

This Rabbit Polyclonal antibody specifically detects SIAH1 in WB. It exhibits reactivity toward Human.

Quick Overview for SIAH1 antibody (ABIN265177)

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SIAH1 Antibody

Specificity: This antibody detects SIAH1 / E3 Ubiquitin Ligase. Antibody tested on human brain lysate.

Purification: Purified

Immunogen: Synthetic peptide derived from the human SIAH-1 protein

Isotype: IgG

Application Details

Application Notes: Western blot (1-5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for SIAH1

Target: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Alternative Name: SIAH1

Background: Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1. Both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a

Gene ID: 6477

NCBI Accession: NP_001006611

UniProt: Q8IUQ4