HDAC9 antibody

Catalog No. ABIN265461

Product Details anti-HDAC9 Antibody

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of HDAC9 protein. (region surrounding Pro1047)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for HDAC9

Target: HDAC9 (Histone Deacetylase 9 (HDAC9))

Alternative Name: HDAC9 (HDAC9 Products)

Synonyms: HD7 antibody, HD7b antibody, HD9 antibody, HDAC antibody, HDAC7 antibody, HDAC7B antibody, HDAC9B antibody, HDAC9FL antibody, HDRP antibody, MITR antibody, AV022454 antibody, D030072B18Rik antibody, HD7B antibody, Hdac7b antibody, Mitr antibody, mKIAA0744 antibody, hdac9 antibody, zgc:73392 antibody, TWIST antibody, HDAC9 antibody, DKFZp459E171 antibody, HDA09 antibody, HISTONE DEACETYLASE antibody, histone deacetylase 9 antibody, RGD1310748 antibody, RGD1563092 antibody, hdac9-A antibody, histone deacetylase 9 antibody, histone deacetylase 9b antibody, histone deacetylase 9 S homeolog antibody, HDAC9 antibody, Hdac9 antibody, hdac9b antibody, HDA9 antibody, hdac9.S antibody

Background: The remodeling of chromatin is a critical component of ranscriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription.Synonyms: HD7B, HD9, HDAC7B, HDRP, Histone deacetylase 9, Histone deacetylase-related protein, KIAA0744, MEF2-interacting transcription repressor MITR, MITR

Molecular Weight: approx. 111, 160 kDa

Gene ID: 9734

NCBI Accession: NP_001191073

UniProt: Q9UKV0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development