Integrin beta 5 antibody

Catalog No. ABIN265481

The Rabbit Polyclonal anti-Integrin beta 5 antibody is suitable to detect Integrin beta 5 in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for Integrin beta 5 antibody (ABIN265481)

Target: Integrin beta 5 (ITGB5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Integrin beta 5 Antibody

Specificity: This antibody antibody detects endogenous levels of Integrin beta5 protein. (region surrounding Val746)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Integrin beta 5

Target: Integrin beta 5 (ITGB5)

Alternative Name: Integrin beta-5 / ITGB5

Background: Integrins are heterodimers composed of noncovalently associated transmembrane alpha and beta subunits. The 16alpha and 8beta subunits heterodimerize to produce more than 20 different receptors. Most Integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain Integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster Integrins by binding to adjacent Integrin receptors, both receptor clustering and ligand occupancy are necessary for the activation of Integrinmediated responses. In addition to mediating cell adhesion and cytoskeletal organization, Integrins function as signaling receptors. Signals transduced by Integrins play a role in many ,biological processes, including cell growth, differentiation, migration and apoptosis.Synonyms: ITGB-5

Molecular Weight: approx. 88 kDa

Gene ID: 3693

NCBI Accession: NP_002204

UniProt: P18084

Pathways: Integrin Complex