NTAN1 antibody

Catalog No. ABIN2660532

The Rat Monoclonal anti-NTAN1 antibody (Clone MECA-79) (ABIN2660532) specifically detects NTAN1 in FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for NTAN1 antibody (ABIN2660532)

Target: NTAN1 (N-terminal Asparagine Amidase (NTAN1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This NTAN1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: MECA-79

Product Details anti-NTAN1 Antibody

Purity: This antibody is at > 85 % purity.

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for NTAN1

Target: NTAN1 (N-terminal Asparagine Amidase (NTAN1))

Alternative Name: PNAd

Background: MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 β1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.