MIF antibody (Biotin)

Catalog No. ABIN2660796

The Mouse Monoclonal anti-MIF antibody is suitable to detect MIF in samples from Human. It has been validated for BCA.

Quick Overview for MIF antibody (Biotin) (ABIN2660796)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MIF antibody is conjugated to Biotin

Application: Biochemical Assay (BCA)

Clone: 10C3

Product Details anti-MIF Antibody (Biotin)

Purification: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: Human macrophage migration inhibitory factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide expressed by multiple cell types, including activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. MIF plays many roles in biological processes such as catalytic activity, immunity, endocrine regulation, signal modulation, and inflammation. MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors. Studies suggest that MIF might serve as a molecular link between chronic inflammation and cancer. Recombinant MIF consists of a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly dimeric and trimeric forms.

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling