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CD25 antibody (FITC)

IL2RA Reactivity: Rat FACS Host: Mouse Monoclonal OX-39 FITC

Catalog No. ABIN2661617

Datasheet as PDF

Target See all CD25 (IL2RA) Antibodies

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Reactivity
165

111

36

10

10

5

3

2

2

2

1

1
Rat
Host
137

80

77

2

1

1
Mouse
Compatible Secondaries
Clonality
222

70

4
Monoclonal
Conjugate
117

33

28

25

22

11

6

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is conjugated to FITC
Application
218

86

54

53

44

44

23

18

15

13

8

8

6

6

4

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Purification

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

Clone

OX-39

Isotype

IgG1 kappa

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IL2RA Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Concentration

0.5 mg/mL

Buffer

Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.5 % BSA .

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Protect from prolonged exposure to light. Do not freeze.

Storage

4 °C

Storage Comment

The antibody solution should be stored undiluted between 2°C and 8°C.
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name

CD25 (IL2RA Products)

Background

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Pathways

JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation