Ki-67 antibody (Pacific Blue)

Catalog No. ABIN2662184

The Pacific Blue-conjugated Rat Monoclonal anti-Ki-67 antibody (Clone 16A8) (ABIN2662184) specifically detects Ki-67 in FACS, IF, ELISA, IP and BR. The antibody is reactive with Human and Mouse samples.

Quick Overview for Ki-67 antibody (Pacific Blue) (ABIN2662184)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is conjugated to Pacific Blue

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: 16A8

Product Details anti-Ki-67 Antibody (Pacific Blue)

Characteristics: The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Purification: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Pathways: Glycosaminoglycan Metabolic Process