SIRPA antibody (PE)

Catalog No. ABIN2663092

The Mouse Monoclonal anti-SIRPA antibody is suitable to detect SIRPA in samples from Rat. It has been validated for FACS, IP and BR.

Quick Overview for SIRPA antibody (PE) (ABIN2663092)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: OX-41

Product Details anti-SIRPA Antibody (PE)

Purification: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.5 % BSA (origin U.S.)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a

Background: Rat CD172a, also known as a signal-regulatory protein α (SIRPα), is a 75-110 kD transmembrane glycoprotein involved in the receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily primarily expressed on monocytes/macrophages, granulocytes, dendritic cells and neurons. CD172a serves as a substrate of activated receptor ttyrosine kinases (RTKs). The interaction of the CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion and phagocytosis. CD47 (IAP) is an extracellular ligand for CD172a.