C3a antibody

Catalog No. ABIN2664084

The Mouse Monoclonal anti-C3a antibody is suitable to detect C3a in samples from Human. It has been validated for IP, ELISA and FACS.

Quick Overview for C3a antibody (ABIN2664084)

Target: C3a (Complement Fragment 3a (C3a))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3a antibody is un-conjugated

Application: Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS)

Clone: K13-16

Product Details anti-C3a Antibody

Purification: The antibody was purified by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for C3a

Target: C3a (Complement Fragment 3a (C3a))

Alternative Name: C3a/C3a/C3

Background: C3, complement component 3, is a 195 kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.

Pathways: Complement System