IL-33 antibody (AA 112-270)

Catalog No. ABIN2665497

This Goat Polyclonal antibody specifically detects IL-33 in IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for IL-33 antibody (AA 112-270) (ABIN2665497)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: Poly5163

Product Details anti-IL-33 Antibody

Binding Specificity: AA 112-270

Cross-Reactivity (Details): In sandwich ELISA, no cross-reactivity with mouse or rat IL-33.

Purification: The antibody was purified by antigen affinity chromatography.

Immunogen: AA 112-270 (Accession# NM_033439) was expressed in E. coli.

Isotype: Ig Fraction, IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Background: IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1α or IL-1β stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.

Pathways: Production of Molecular Mediator of Immune Response