IL-33 antibody (Ser109)

Catalog No. ABIN2665498

The Goat Polyclonal anti-IL-33 antibody is suitable to detect IL-33 in samples from Mouse and Rat. It has been validated for WB, IHC, IP and IF.

Quick Overview for IL-33 antibody (Ser109) (ABIN2665498)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: Poly5165

Product Details anti-IL-33 Antibody

Binding Specificity: Ser109

Cross-Reactivity: Rat (Rattus)

Purification: The antibody was purified by antigen affinity chromatography.

Immunogen: Ser109-Isoleucin266 (Accession# NM_133775) was expressed in E. coli.

Isotype: Ig Fraction, IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Background: IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

Pathways: Production of Molecular Mediator of Immune Response