CDK1 antibody

Catalog No. ABIN2666269

The Mouse Monoclonal anti-CDK1 antibody is suitable to detect CDK1 in samples from Human, Rat, Monkey, Cow, Xenopus laevis and Drosophila melanogaster. It has been validated for FACS.

Quick Overview for CDK1 antibody (ABIN2666269)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human, Rat, Monkey, Cow, Xenopus laevis, Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: POH-1

Product Details anti-CDK1 Antibody

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Xenopus laevis, Fruit Fly (Drosophila melanogaster)

Purification: This antibody was purified by affinity chromatography.

Isotype: IgG2a, IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.4, containing 0.09 % sodium azide at 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store undiluted between 2°C and 8°C.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: Cdc2

Background: Cdc2 (p34), also known as cell division cycle 2, G1 to S and G2 to M, cyclin dependent kinase 1, p34 kinase, and CDK1, is serine/threonine kinase that is ubiquitously expressed in eukaryotes. Cdc2 is found as both a cytoplasmic and nuclear protein. cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally in eukaryotes. The cdc2 protein is highly phosphorylated on threonine, serine, and tyrosine, tyrosine phosphorylation is subject to cell cycle regulation. Cdc2 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. The POH-1 antibody recognizes human, monkey and bovine cdc2 and is useful for Western blotting. The POH-1 antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (formalin-fixed, paraffin embedded), and immunofluorescence.

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA